Rice microtubule-associated protein OsMAP65-3.1, but not OsMAP65-3.2, plays a critical role in phragmoplast microtubule organization in cytokinesis

Rice microtubule-associated protein OsMAP65-3.1, but not OsMAP65-3.2, plays a critical role in phragmoplast microtubule organization in cytokinesis

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In plants, MAP65 preferentially cross-links the anti-parallel microtubules (MTs) and hellfire sloe gin plays an important role for cytokinesis.However, the functions of MAP65 isoforms in rice (Oryza sativa.L) are largely unknown.Here, we identified two MAP65-3 homologs in rice, OsMAP65-3.

1 and OsMAP65-3.2.We found that both OsMAP65-3.1 and OsMAP65-3.

2 were similar in dimerization and location to AtMAP65-3, and the expression of either rice genes driven by the AtMAP65-3 promoter suppressed the cytokinesis failure and growth defect of atmap65-3.However, OsMAP65-3.1 with native promoter also recovered the atmap65-3, but OsMAP65-3.2 with its own promoter had no effects.

OsMAP65-3.1 but merlot redbud tree for sale not OsMAP65-3.2 was actively expressed in tissues enriched with dividing cells.R1R2R3-Myb (MYB3R) transcription factors directly bound to the OsMAP65-3.

1 promoter but not that of OsMAP65-3.2.Furthermore, osmap65-3.2 had no obvious phenotype, while either osmap65-3.

1 or osmap65-3.1(+/-) was lethal.The eminent MTs around the daughter nuclei and cytokinesis defects were frequently observed in OsMAP65-3.1-defective plants.

Taken together, our findings suggest that OsMAP65-3.1, rather than OsMAP65-3.2, plays essential roles in rice cytokinesis resulting from their differential expression which were passably directly regulated by OsMYB3Rs.